A second person has died in hospital following a fatal collision in Dimboola on 3 March.

It is alleged a Toyota Hilux towing a hay baler and a Mazda sedan collided on the Western Highway about 5 pm.

The driver of the Mazda, an 80-year-old Nhill woman, died at the scene.

Her passenger, an 85-year-old Nhill man, was airlifted to hospital with serious injuries and died on 10 March.

The driver of the Toyota, a 58-year-old man from South Australia, was taken to hospital with minor injuries.

The man has since been arrested and charged with dangerous driving causing death, careless driving and other traffic-related offences.